Professor Udai's group uses a range of platforms that measure proteins including MSD, Luminex, Nanostring and immunofluorescence. Examples of assays developed and used in clinical trials include p-AKT, p-GSK3B in platelet-rich plasma, p-PRAS40 in hair follicles, p-CHK1 in peripheral blood mononuclear cells and gamma H2AX in hair follicles by immunofluorescence. In addition, these tests are optimised for tumour tissue and have been used in multiple phase I clinical trials.
The group validates these assays to a high standard, with special attention to dynamic ranges and reproducibility of assays in addition to the stability of samples. The team has an extensive list of internal standard operating procedures and runs quality control checks as per Good Clinical Practice.