Centre for In Vivo Modelling Service Core

At the Centre for In Vivo Modelling (CIVM), we combine advanced animal genetics and cutting-edge technologies to drive cancer research. Our multidisciplinary team specialises in the generation and maintenance of genetically engineered mouse models (GEMMs), humanised mouse strains, and patient-derived models (xenografts and organoids), using innovations such as CRISPR gene editing, embryo manipulation, and in vivo genetic screening. We develop and cryopreserve new cancer models that closely replicate human disease, supporting translational studies that inform effective therapies. Our approach integrates rigorous scientific standards, ethical oversight, and collaborative expertise, aiming to accelerate progress in understanding cancer biology and developing better treatments for patients.

Our Centre is dedicated to driving innovation and excellence in cancer research through advanced in vivo modelling. We work in close collaboration with the ICR researchers and clinicians at The Royal Marsden to generate genetically engineered mouse models (GEMMs) and patient-derived models, such as patient-derived xenografts (PDXs) and patient-derived organoids (PDOs) to interrogate cancer biology in its own ecosystem. By leveraging these sophisticated in vivo systems, the Centre aims to:

  • Develop innovative cancer models in collaboration with ICR researchers to advance cancer research and drug discovery.
  • Work in partnership with The Royal Marsden Hospital to obtain patient samples and generate new patient-derived cancer models for translational studies.
  • Foster close interdisciplinary collaboration with drug discovery teams to leverage these in vivo models in the creation and testing of next-generation anti-cancer therapies.
  • Continuously improve the sophistication and relevance of our cancer models, ensuring they more faithfully recapitulate the complexity of human disease and enhance the translational impact of our research.

 

Our services

Advantages of cryopreserving your strains:

  • Allows you to save space, by getting the mice you need, when you need;
  • Reduces your animal costs;
  • Reduces animal use;
  • Reduces risk from disasters (e.g. disease outbreaks, breeding cessation, equipment failures, genetic contamination, natural disasters, etc…).

 What can be cryopreserved?

  • Mouse Sperm
  • Mouse Embryos
  • Mouse Embryonic Stem Cells
  • Mouse Oocytes

 Sperm Cryopreservation:

Description: Sperm is retrieved from the epididymal tissues of 3 male mice and is cryopreserved in 20 to 30 straws that are stored in liquid-phase, liquid nitrogen across two tanks in two separate locations (SRD and CCDD), to ensure sample safety and mitigate risks associated to unexpected or uncontrollable events.

Material needed: 3 males, reproductively active, 12-25 weeks old

Timeline: 2-6 weeks (dependant on QC method of choice)

Considerations: this method of cryopreservation is rapid and cheap; however, it only preserves half of the genome. This method is only recommended for single mutations on a common inbred background.

Quality Control: we provide different levels of Quality Control (QC) for different price ranges.

  1. Test thaw QC: we will thaw 1 straw the day after cryopreservation and visually assess motility and viability of the recovered sperm
  2. IVF and culture to blastocyst QC: we highly recommend this QC step. In addition to test thaw, we will also perform IVF and culture embryos up to blastocyst stage. We will provide the investigator with a fertility rate (%) for the recovered sperm. We will charge an extra cost to cover the IVF procedure.
  3. IVF and embryo transfer QC: In addition to test thaw, we will perform IVF and transfer 2-cell embryos into up to 3 pseudopregnant females to generate viable embryos/live pups. We will charge an extra cost to cover the IVF and embryo transfer procedures.

    Please note that we require you to provide your genotyping protocol, as well as full detail of the genetic content of each strain that you submit for cryopreservation.

Diagram of Sperm Cryopreservation

Embryo Cryopreservation:

Description: Female mice are hormonally superovulated and oocytes are retrieved for in vitro fertilisation (IVF) with sperm from donor male. Resulting embryos are placed in cryoprotectant and loaded into multiple straws, which are gradually cooled and stored in liquid-phase liquid nitrogen in two separate tanks.

Material needed: Donor male and 8-10 donor females

Timeline: 12-15 weeks

Diagram of Embryo Cryopreservation

Embryonic Stem Cells Cryopreservation:

Not available, yet.

Oocyte Cryopreservation:

Not available, yet.

Cryostorage:

If you have cryopreserved mouse sperm/embryo/oocytes at another institution, we can cryostorage your samples for an annual fee. We do require that the investigator takes charge of shipping costs into our facility, and that thawing and genotyping protocols are submitted to the CIVM.

The CIVM stores all samples in liquid-phase liquid nitrogen tanks (CryoPlus1, ThermoFisher Scientific). Material retrieved from each strain is split between 2 tanks, a main and a backup tank, for redundancy. For additional safety, these 2 tanks are located in two separate buildings at ICR Sutton. Both tanks are continuously monitored by T-scan alarm systems and undergo annual service, as well as daily visual inspections.


 

Sperm Cryorecovery:

Description: Frozen sperm is cryorecovered by IVF, followed by embryo transfer. We can purchase wild-type female oocyte donors of the same genetic background, or alternatively the investigator can provide homozygous oocyte donors of the same strain.

Material needed: straw with frozen sperm and 8 to 12 females for IVF, 7-16 weeks old.

Timeline: 12-15 weeks

Diagram of Sperm Cryorecovery

 

Embryo Cryorecovery:

Description: Frozen 2-cell embryos are thawed and transferred into pseudopregnant females.

Material needed: straw(s) with frozen 2-cell embryos

Timeline: 8-10 week


Oocyte Cryorecovery:

Not available, yet.

 

Mouse rederivation

Description: Mouse rederivation is a process used to produce pathogen-free mouse colonies by removing microbial contaminants from existing lines. The procedure can be performed either through natural mating or in vitro fertilization (IVF):

  • In natural mating, embryos are obtained from donor mice and transferred into pathogen-free recipient females.
  • In IVF-based rederivation, fertilized embryos are created in vitro using gametes from donor mice and then implanted into clean recipient females.

Both methods effectively eliminate pathogens, allowing safe importation of mouse strains from lower health-status facilities into the ICR BSU. Samples from both litter and recipient mother will be sent for Health Screening and the associated costs will be charged separately to the Investigator.

Material needed: For IVF-based rederivation we require the investigator to provide 2 males, reproductively active, 12-25 weeks old, and the CIVM will purchase wild-type female egg-donors. Alternatively, if maintaining homozygosity is essential, the investigator will need to provide additional 6-10 females, 7-16 weeks old.

Timeline: 12-15 week

Mouse Rederivation Mating Diagram

Mouse Rederivation IVF diagram

We are currently setting up CRISPR/Cas9-based gene editing protocols. Soon, you’ll be able to apply for projects that involve developing new alleles based on:

  • Knockout by indel formation
  • Knockout by precise deletion
  • Conditional knockout
  • Knock-in of point mutations
  • Knock-in of small tags
  • Large knock-in
  • Exon replacement

These alleles will be developed based on Electroporation of Microinjection of CRISPR/Cas9 system reagents.

We will collaborate with you to design the best strategy and help you generate the genetically engineered mice you need for your project. 

We also provide:

  • Development of humanised mouse strains
  • Development of Patient-derived xenografts (PDX) and organoid models

Latest ICR News

24/02/26

Researchers from The Institute of Cancer Research, London, attended a prestigious ceremony at St James’s Palace today  to mark the formal presentation of the Queen Elizabeth Prize for Higher and Further Education (previously known as the Queen’s Anniversary Prize).

The national honour recognises excellence, innovation and demonstrable benefit in UK higher and further education. It is the country’s highest civilian award for achievement in this field, celebrating the profound impact that UK institutions can have on society.

This year, The Institute of Cancer Research (ICR) was honoured for its sustained, world-leading contributions to radiotherapy research and education, which have shaped clinical practice, driven innovation across disciplines and improved outcomes for patients around the world.

The award acknowledges the ICR’s long‑standing commitment to integrating scientific discovery with training and skills development – ensuring that radiotherapy research remains at the cutting edge, shaping global practice while nurturing the next generation of scientific leaders.

It also marks the third such prize for the ICR. The institute was previously recognised in 2017 for its outstanding contribution to the discovery of new cancer drugs and in 2023 for its innovative work in breast cancer research.

A tribute to radiotherapy research and teaching at the ICR

The ICR’s award reflects more than a century of pioneering discoveries in radiotherapy, often in collaboration with the ICR’s clinical partner, The Royal Marsden NHS Foundation Trust. The institute has had a central role in transforming radiotherapy from a relatively rudimentary, imprecise treatment into a highly targeted, sophisticated and personalised modality that is now used to treat millions of patients worldwide.

Recent breakthroughs from ICR scientists have included new approaches both to adapting radiotherapy plans during treatment as tumours move or shrink and to understanding biological responses to radiotherapy. Alongside this, their work has led to improvements in proton beam therapy targeting and next generation imaging tools that detect subtle changes in tumour biology. The ICR has also led many practice-changing clinical trials that have made treatment regimens more efficient, reducing hospital visits for patients and lowering costs for the NHS.

These achievements build on the ICR’s long legacy in radiotherapy, which includes discoveries that laid the foundations for intensity-modulated radiotherapy, stereotactic radiotherapy and radiosensitising drugs.

These innovations have helped ensure that radiotherapy remains one of the most potent and commonly used cancer treatments. Today, four in every 10 cancer patients who are cured of their disease will have received radiotherapy as part of their treatment.

Today’s recognition also celebrates the ICR’s unique educational environment – one in which biologists, physicists, chemists, computer scientists and clinicians work side by side, combining their expertise to propel radiotherapy research forwards. This multidisciplinary approach is embedded in everything from postgraduate training and clinical fellowships to specialty academic training for NHS partners.

Celebration at St James’s Palace

The ceremony at St James’s Palace brought together leaders from across education, science, medicine, engineering and public life. Representatives from prize-winning institutions met with members of the Royal Family, who congratulated the recipients and spoke about the importance of world-leading education and training to the UK’s future prosperity.

Held in the Palace’s historic State Rooms , the event highlighted the breadth of disciplines recognised through the prize cycle. This year’s recipients, including the ICR, showcased how higher and further education can deliver direct benefits to society through technological innovation, cultural enrichment, improved public health and global knowledge exchange.

Guests also had the opportunity to meet fellows, students, apprentices and early career researchers who have benefited from the programmes, courses and training initiatives recognised by the prizes.

Recognising outstanding innovation in higher education

The Queen Elizabeth Prizes for Higher and Further Education form part of the UK national honours system and are presented biennially by a senior member of the Royal Family. First awarded in 1994, the prizes are granted every two years by the Sovereign on the advice of the Prime Minister following a rigorous and independent process of review carried out by the Royal Anniversary Trust, an independent charity.

The Prizes are awarded to universities and colleges across the UK whose work demonstrates excellence, originality and wide-reaching societal benefit. Crucially, the prizes recognise the institution as a whole, rather than individual researchers – highlighting collective achievement across departments, professional services and education teams.

Much of the research recognised by the award was funded by the ICR, which is both a research institute and a charity. Additional financial support was provided by Cancer Research UK and various government funding bodies – including the National Institute for Health and Care Research (NIHR) Biomedical Research Centre at The Royal Marsden and the ICR and the Medical Research Council, which funded the installation of the MR Linac with a £10 million grant to the ICR.

Looking ahead: a continuing legacy of discovery and learning

Receiving the Queen Elizabeth Prize marks not an endpoint, but a milestone in the ICR’s mission to defeat cancer. It serves both as a tribute to the breakthroughs that have already transformed radiotherapy and as a springboard for the next generation of discoveries.

Researchers at the ICR continue to explore new frontiers in radiotherapy, including novel radiotherapy-drug combinations, precision radiotherapy guided by advanced imaging, and personalised treatment plans that maximise effectiveness while limiting side effects.

With an ever-increasing number of new tools at their disposal – including artificial intelligence, machine learning–based image analysis, new particle beam technologies and biological profiling of tumours – researchers are poised to develop even more personalised and effective radiotherapy strategies.

These research aims are supported by a strong educational strategy designed to equip future clinicians and researchers with the knowledge, skills and collaborative mindset needed to accelerate progress.

A proud day for the ICR

Today’s ceremony at St James’s Palace was a moment of celebration and pride for the ICR. In officially recognising the ICR’s role as a world-leading institution at the intersection of research and education, it fortified the institute’s ongoing mission to make the discoveries that defeat cancer.

Professor Kevin Harrington, Head of the Division of Radiotherapy and Imaging at the ICR, said:

“We are deeply honoured to receive the Queen Elizabeth Prize for Higher and Further Education. Radiotherapy is one of the most important tools we have for treating cancer, and this award recognises generations of scientists, clinicians, educators and technical specialists whose combined efforts have helped redefine what this treatment can achieve.

“At the ICR, we have always believed that combining disciplines is the key to major breakthroughs, and radiotherapy is one of the clearest examples of that. Today’s prize reflects the power of innovation when different areas of science come together with a shared goal: to give people with cancer better outcomes and a better quality of life.”

Professor Kristian Helin, Chief Executive of the ICR, said:

“We are incredibly proud to accept this national honour, which is a testament to the extraordinary dedication of our staff and collaborators, and to the positive impact their work has on people with cancer around the world.

“Our mission has always been driven by the conviction that pioneering research must go hand in hand with excellence in training. We are committed to creating an environment where scientific discovery directly informs education – providing students, researchers and clinicians with the skills to deliver tomorrow’s breakthroughs.

“While the challenges ahead are considerable, so too are the opportunities. Radiotherapy will continue to evolve rapidly, and with the right support, we can continue to deliver treatments that are increasingly precise, personalised and effective for patients.”

Celebrating and championing work across the UK

This year’s ceremony continued the long tradition of celebrating UK institutions that have made unique contributions to national and global life. Over the past 30 years, the prizes have recognised achievements across a wide range of fields, including health and medicine, pure and applied science, engineering, the arts, education, social sciences and environmental research.

The current round of the awards – the 16th – saw a total of 19 prizes being announced on 25 November 2025. 

Science Minister, Lord Patrick Vallance, said: 

"Cancer touches millions of lives, from those living with it to those caring for others. It is vital that we continue to support work which advances treatment and minimises side effects. This pioneering and globally important work from the ICR highlights how British research expertise backed by government funding is delivering real change and support for people who are going through the most difficult period of their life."

Sir Damon Buffini, Chair of the Royal Anniversary Trust, said:

“The Queen Elizabeth Prizes for Higher and Further Education celebrate the power of education to change the world for the better. This much-loved national honour recognises, at the highest level of state, outstanding work in UK universities and colleges, and the remarkable benefit they bring to our economy, society and the wider world.”

Prime Minister Sir Keir Starmer congratulated the prize-winners, saying:

“Your work is improving lives, growing our economy and helping to shape a fairer, more prosperous future. I would like to thank all the winners commended this year for their commitment, creativity and contribution to our nation.”