(2019). Autoinhibition Mechanism of the Ubiquitin-Conjugating Enzyme UBE2S by Autoubiquitination. Structure,
(2019). Degron-tagged reporters probe membrane topology and enable the specific labelling of membrane-wrapped structures. Nature communications,
(2018). Constitutive regulation of mitochondrial morphology by Aurora A kinase depends on a predicted cryptic targeting sequence at the N-terminus. Open biology,
Aurora A kinase (AURKA) is a major regulator of mitosis and an important driver of cancer progression. The roles of AURKA outside of mitosis, and how these might contribute to cancer progression, are not well understood. Here, we show that a fraction of cytoplasmic AURKA is associated with mitochondria, co-fractionating in cell extracts and interacting with mitochondrial proteins by reciprocal co-immunoprecipitation. We have also found that the dynamics of the mitochondrial network are sensitive to AURKA inhibition, depletion or overexpression. This can account for the different mitochondrial morphologies observed in RPE-1 and U2OS cell lines, which show very different levels of expression of AURKA. We identify the mitochondrial fraction of AURKA as influencing mitochondrial morphology, because an N-terminally truncated version of the kinase that does not localize to mitochondria does not affect the mitochondrial network. We identify a cryptic mitochondrial targeting sequence in the AURKA N-terminus and discuss how alternative conformations of the protein may influence its cytoplasmic fate..
(2018). An E2-ubiquitin thioester-driven approach to identify substrates modified with ubiquitin and ubiquitin-like molecules. Nature communications,
(2018). Conditional control of fluorescent protein degradation by an auxin-dependent nanobody. Nature communications,
(2017). Quantitative Cell Cycle Analysis Based on an Endogenous All-in-One Reporter for Cell Tracking and Classification. Cell reports,
(2017). PLK1 Activation in Late G2 Sets Up Commitment to Mitosis. Cell reports,
Ahmed Alfar, E.
(2017). Distinct Levels of Reactive Oxygen Species Coordinate Metabolic Activity with Beta-cell Mass Plasticity. Scientific reports,
(2016). RETRACTED: The Anaphase-Promoting Complex/Cyclosome Is Essential for Entry into Meiotic M-Phase. Developmental cell,
(2016). Retraction Notice to: The Anaphase-Promoting Complex/Cyclosome Is Essential for Entry into Meiotic M-Phase. Dev cell,
Di Fiore, B.
(2015). The ABBA Motif Binds APC/C Activators and Is Shared by APC/C Substrates and Regulators. Developmental cell,
(2015). Real-time deformability cytometry: on-the-fly cell mechanical phenotyping. Nature methods,
(2015). A Human Interactome in Three Quantitative Dimensions Organized by Stoichiometries and Abundances. Cell,
(2011). APC15 drives the turnover of MCC-CDC20 to make the spindle assembly checkpoint responsive to kinetochore attachment. Nature cell biology,
(2010). Pom121 links two essential subcomplexes of the nuclear pore complex core to the membrane. The journal of cell biology,
Nuclear pore complexes (NPCs) control the movement of molecules across the nuclear envelope (NE). We investigated the molecular interactions that exist at the interface between the NPC scaffold and the pore membrane. We show that key players mediating these interactions in mammalian cells are the nucleoporins Nup155 and Nup160. Nup155 depletion massively alters NE structure, causing a dramatic decrease in NPC numbers and the improper targeting of membrane proteins to the inner nuclear membrane. The role of Nup155 in assembly is likely closely linked to events at the membrane as we show that Nup155 interacts with pore membrane proteins Pom121 and NDC1. Furthermore, we demonstrate that the N terminus of Pom121 directly binds the β-propeller regions of Nup155 and Nup160. We propose a model in which the interactions of Pom121 with Nup155 and Nup160 are predicted to assist in the formation of the nuclear pore and the anchoring of the NPC to the pore membrane..
(2009). UBE2S elongates ubiquitin chains on APC/C substrates to promote mitotic exit. Nature cell biology,
(2007). The actin multigene family of Paramecium tetraurelia. Bmc genomics,
(2006). The Conserved Transmembrane Nucleoporin NDC1 Is Required for Nuclear Pore Complex Assembly in Vertebrate Cells. Molecular cell,
(2006). A Multigene Family Encoding R-SNAREs in the Ciliate Paramecium tetraurelia. Traffic,
DNA damage during S-phase mediates the proliferation-quiescence decision in the subsequent G1 via p21 expression. Nature communications,
A broad spectrum of actin paralogs in Paramecium tetraurelia cells display differential localization and function. Journal of cell science,