The genomics facility within the TPU works under Good Clinical and Laboratory Practice (GCLP) and ISO 15189:2012 guidelines to provide next-generation sequencing of tumours. The unit has a strong focus on cancer biology and clinical treatment, and its experimental workflows include the ability to sequence and analyse formalin-fixed paraffin embedded samples (FFPE), minimal amounts of DNA/RNA samples (30–50 ng), analysis of tumour clonal heterogeneity, cfDNA and drug resistance.
The TPU has the capacity to undertake large projects (ExomeSeq and WGS) and smaller-scale projects, such as target resequencing and analysis of amplicons. It uses a dedicated 480-core, high-performance computing cluster to analyse sequencing data. Automated analysis pipelines are available for whole genome, exome and transcriptome sequencing, built around standard, open-source software. The bioinformatics team at the TPU also has the experience necessary to perform custom analyses and integration of sequencing data sets.